Elizabeth I
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Gender: Female
Elizabeth I is a queen of England and a monarch from the Tudor dynasty. In 1565, she battles her first cousin once removed, Mary Stuart, over the throne. Her betrayal of Bruford and Tarkus, two retainers of Mary, led to the two knights cursing humanity; Dio Brando uses this resentment to his advantage over three hundred years later when he revives them as zombies.
(Source: JoJo's Bizarre Adventure Wiki)
